一种建立优良玉米自交系农系531高效再生体系的方法

Method for building high-efficiency regeneration system of superior corn self-bred line agriculture line 531

Abstract

本发明公开了一种建立优良玉米自交系农系531高效再生体系的方法,属于植物基因工程和转基因育种领域。本发明以农系531的幼胚为外植体,在愈伤组织诱导培养基中进行诱导,产生II型胚性愈伤组织,将II型胚性愈伤组织在胚状体诱导培养基中进行光下胚状体诱导,产生绿色胚状体,再将绿色胚状体转移至再生培养基中,在光下培养成再生植株,然后在生根培养基中进行生根诱导、Hogland营养液中进行炼苗,使再生植株的根部长出新的粗壮的根,再移栽至营养土中缓苗培养,最后移栽至大田中,即可正常生长结实。该再生技术适用的是应用价值很高的优良自交系玉米农系531,在玉米转基因育种过程中可以使农系531玉米的优良品质得以遗传,对功能基因组研究也具有重要意义。
The invention discloses a method for building a high-efficiency regeneration system of superior corn self-bred line agriculture line 531, belonging to the field of plant genetic engineering and transgenosis breeding. The invention takes an agriculture line 531 rataria as an explant, induces in a callus induction medium and produces an II-type embryonic callus; the II-type embryonic callus is subjected to embryoid induction under light in an embryoid induction medium to produce a green embryoid; then, the green embryoid is transported to a regeneration medium and is cultured into a regeneration plant under light; root induction is carried out in a rooting medium, and acclimatization is carried out in Hogland nutrient solution to ensure that a new thick root grows on the root of the regeneration plant; the root is transplanted to nutritional soil for rejuvenation culture; and finally, the root is transplanted to a land for growing field crops to normally grow and seed. The regeneration technology is suitable for the superior corn self-bred line agriculture line 531 with high application value, can ensure that the superior quality of agriculture line 531 corn can be inherited in the corn transgenosis breeding process, and has an important meaning for functional genome group research.

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Cited By (8)

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    CN-102217533-AOctober 19, 2011中国农业大学一种制备玉米Ⅱ型胚性愈伤组织的方法
    CN-102217533-BDecember 26, 2012中国农业大学Method for preparing corn type II embryogenic calli
    CN-102326513-AJanuary 25, 2012新疆康地种业科技股份有限公司一种建立通用玉米再生体系的方法
    CN-103444525-ADecember 18, 2013广东省农业科学院作物研究所一种无激素的玉米愈伤组织再生方法
    CN-103444525-BDecember 24, 2014广东省农业科学院作物研究所Hormone-free corn callus regeneration method
    CN-103477980-AJanuary 01, 2014河南农业大学Young embryo callus induction and plant regeneration method for pop corns
    CN-104130058-ANovember 05, 2014吉林省农业科学院High-efficiency inducing subculture medium for young corn embryos and preparation method thereof
    CN-104130058-BAugust 17, 2016吉林省农业科学院一种玉米幼胚高效诱导继代培养基及制备方法