Isocitric acid determination reagent (kit) and isocitric acid concentration determination method



The invention relates to an isocitric acid determination reagent (kit) which uses an enzyme multiplication method, an enzyme colorimetric method and an ELISA technology, and simultaneously relates to an isocitric acid concentration determination method as well as the composition and the components of the reagent, which belong to the technical field of food inspection and determination. The main components of the reagent (kit) of the invention comprise: buffer solution, coenzyme, alanine, isocitrate dehydrogenase, glutamic-pyruvic transaminase, glutamate dehydrogenase and stabilizer; a sample is mixed with the reagent in a certain volume ratio, so that the sample and the reagent carry out a series of enzymatic reactions; and then the reactant is put under an UV/visible light analyzer, the increase of light absorbency at 340nm of a main wave length is detected, so as to measure the concentration of isocitric acid.




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