Isocitric acid determination reagent (kit) and isocitric acid concentration determination method

异柠檬酸测定试剂(盒)及异柠檬酸浓度测定方法

Abstract

The invention relates to an isocitric acid determination reagent (kit) which uses an enzyme multiplication method, an enzyme colorimetric method and an ELISA technology, and simultaneously relates to an isocitric acid concentration determination method as well as the composition and the components of the reagent, which belong to the technical field of food inspection and determination. The main components of the reagent (kit) of the invention comprise: buffer solution, coenzyme, alanine, isocitrate dehydrogenase, glutamic-pyruvic transaminase, glutamate dehydrogenase and stabilizer; a sample is mixed with the reagent in a certain volume ratio, so that the sample and the reagent carry out a series of enzymatic reactions; and then the reactant is put under an UV/visible light analyzer, the increase of light absorbency at 340nm of a main wave length is detected, so as to measure the concentration of isocitric acid.
本发明涉及一种利用酶倍增法、酶比色法及酶联法技术的异柠檬酸测定试剂(盒),同时本发明还涉及测定异柠檬酸浓度的方法、试剂的组成及成分,属于食品检验测定技术领域。本发明的试剂(盒)主要成分包括:缓冲液、辅酶、丙氨酸、异柠檬酸脱氢酶、谷丙转氨酶、谷氨酸脱氢酶及稳定剂;通过将样品与试剂按一定的体积比混合,使之发生一系列的酶促反应,再将反应物置于紫外/可见光分析仪下,检测主波长340nm处吸光度上升的程度,从而测算出异柠檬酸的浓度大小。

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